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Biochemistry
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JoVE Journal Biochemistry
An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model
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An In-House-Built and Light-Emitting-Diode-Based Photodynamic Therapy Device for Enhancing Verteporfin Cytotoxicity in a 2D Cell Culture Model

Article DOI: 10.3791/64391-v • 11:04 min • January 13th, 2023
January 13th, 2023 •
1, 1, 1, 1, 1, 2,3, 2, 3, 2, 2, 1, 1
1Graduate Program in Pharmaceutical Sciences, Laboratory of Cancer Drug Resistance, Federal University of Parana, 2Graduate Program in Pharmaceutical Sciences, Federal University of Parana, 3Federal Institute of Parana

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Here, we describe a novel, simple, and low-cost device to successfully perform in vitro photodynamic therapy (PDT) assays using two-dimensional HeLa cell culture and verteporfin as a photosensitizer.

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Biochemistry Photodynamic Therapy (PDT) Verteporfin Cytotoxicity 2D Cell Culture Model Device Enhancing Efficacy Photosensitizer Access Expensive Reproducible In Vitro Models Novel Simple Low-cost PDT Assays Cell Cultures PhotoAct Construction Medium Density Fiber Boards (MDF) Dimensions Boxes Barrel Jack Connector Electric Cables Black Ink LED Tapes Brightness Sensor Control Unit Power Button Potentiometers Time Start Touchpad LCD Buzzer Power Supply
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