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JoVE Journal Chemistry
Mikroprøveudtagning i målrettet massespektrometribaseret proteinanalyse af proteiner med lav overflod
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Mikroprøveudtagning i målrettet massespektrometribaseret proteinanalyse af proteiner med lav overflod

Article DOI: 10.3791/64473-v • 10:21 min • January 13th, 2023
January 13th, 2023 •
1, 1, 1, 1
1Department of Pharmacy, University of Oslo

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Der fremlægges en protokol til bestemmelse af biomarkører med lav forekomst fra tørrede serumprøver eksemplificeret med biomarkøren progastrinfrigivende peptid (ProGRP). Antistofbelagte magnetiske perler anvendes til selektiv oprydning og berigelse af et proteotypisk ProGRP-peptid. Det fangede peptid analyseres efterfølgende ved væskekromatografi-tandem massespektrometri.

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Tilbagetrækning udgave 191 DSS immunoaffinitetsoprydning LC-MS / MS mikroprøveudtagning proteinanalyse biomarkører bioanalyse magnetiske perler progastrinfrigivende peptid
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