Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / Determination of S-Phase Duration Using…
JoVE Journal
Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology
Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae
A Strategy for Sensitive, Large Scale Quantitative Metabolomics
Journal (Biology)
A Strategy for Sensitive, Large Scale Quantitative Metabolomics
Isolation of CD146+ Resident Lung Mesenchymal Stromal Cells from Rat Lungs
Journal (Biology)
Isolation of CD146+ Resident Lung Mesenchymal Stromal Cells from Rat Lungs
 
Click here for the English version

Determination of S-Phase Duration Using 5-Ethynyl-2'-deoxyuridine Incorporation in Saccharomyces cerevisiae

Article DOI: 10.3791/64490-v • 08:40 min • October 21st, 2022
October 21st, 2022 •
1, 1, 1
1Institut de Génétique Moléculaire de Montpellier, Université de Montpellier, CNRS

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

We describe two complementary protocols to accurately determine S-phase duration in S. cerevisiae using EdU, a thymidine analog, which is incorporated in vivo and detected using Click chemistry by microscopy and flow cytometry. It allows for the easy characterization of the duration of DNA replication and overlooked replication defects in mutants.

Tags

S-phase Duration 5-Ethynyl-2'-deoxyuridine Incorporation Saccharomyces Cerevisiae Synchronized Budding Yeast Cells Cell Cycle Regulation Nicolas Talarek Juan De Dios Barba Tena SC Medium Overnight Culture Cell Concentration Orbital Agitation Alpha Factor G1 Arrest Light Microscope Centrifuge Supernatant Resuspend
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter