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Immunology and Infection

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JoVE Journal Immunology and Infection
Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes
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Differentiation of Functional Osteoclasts from Human Peripheral Blood CD14+ Monocytes

Article DOI: 10.3791/64698-v • 11:52 min • January 27th, 2023
January 27th, 2023 •
*1, *1, 1, 1
1School of Infection & Immunity, University of Glasgow
* These authors contributed equally

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Summary
Automatic Translation

Osteoclasts are key bone-resorbing cells in the body. This protocol describes a reliable method for the in vitro differentiation of osteoclasts from human peripheral blood monocytes. This method can be used as an important tool to further understand osteoclast biology in homeostasis and in diseases.

Tags

Osteoclast Differentiation CD14+ Monocytes In Vitro Method Functionality High Numbers Of Osteoclasts Arthritis Therapeutic Compounds Screening Patricia Riedlova PBMCs Isolation Density Gradient Medium
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