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Real-Time Detection of Reactive Oxygen Species Production in Immune Response in Rice with a Chemiluminescence Assay

Article DOI: 10.3791/64776-v • 05:44 min • November 25th, 2022
November 25th, 2022 •
1,2, 1,2, 1,2, 3, 1,2
1Shanghai Collaborative Innovation Center of Agri-Seeds, School of Agriculture and Biology, Shanghai Jiao Tong University, 2Joint Center for Single Cell Biology, School of Agriculture and Biology, Shanghai Jiao Tong University, 3Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education), Shanghai Jiao Tong University

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Here, we describe a method for the real-time detection of apoplastic reactive oxygen species (ROS) production in rice tissues in pathogen-associated molecular pattern-triggered immune response. This method is simple, standardized, and generates highly reproducible results under controlled conditions.

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Real-time Detection Reactive Oxygen Species Immune Response Rice Chemiluminescence Assay L-012 PAMP Elicitation Optimized Method Standardized Reproducible Sterilize Dehusked Rice Seeds Ethanol Sodium Hypochlorite Sterile Water Rice Sheath Method Murashige And Skoog Medium Leaf Disc Method MS Plates Growth Matrix Growth Room Photo Period Sheath Segments Microtiter Plate Double Distilled Water Leaf Discs
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