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Visualisierung von Organellen in situ mittels Kryo-STEM-Tomographie
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Visualisierung von Organellen in situ mittels Kryo-STEM-Tomographie

Article DOI: 10.3791/65052-v • 08:37 min • June 23rd, 2023
June 23rd, 2023 •
1, 1, 2, 1
1Department of Chemical and Biological Physics, Weizmann Institute of Sciences, 2Department of Chemical Research Support, Weizmann Institute of Sciences

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Die Kryo-STEM-Tomographie bietet eine Möglichkeit, Organellen intakter Zellen ohne Einbettung, Schnitt oder andere invasive Präparate sichtbar zu machen. Die erzielte 3D-Auflösung liegt derzeit im Bereich von wenigen Nanometern, mit einem Sichtfeld von mehreren Mikrometern und einer zugänglichen Dicke in der Größenordnung von 1 μm.

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Biologie Heft 196
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