Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Enrichment of Native and Recombinant…
JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content.

JoVE Journal Immunology and Infection
Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria
Expanding Cytotoxic T Lymphocytes from Umbilical Cord Blood that Target Cytomegalovirus, Epstein-Barr Virus, and Adenovirus
Journal (Immunology and Infection)
Expanding Cytotoxic T Lymphocytes from Umbilical Cord Blood that Target Cytomegalovirus, Epstein-Barr Virus, and Adenovirus
Co-staining Blood Vessels and Nerve Fibers in Adipose Tissue
Journal (Immunology and Infection)
Co-staining Blood Vessels and Nerve Fibers in Adipose Tissue
 

Enrichment of Native and Recombinant Extracellular Vesicles of Mycobacteria

Article DOI: 10.3791/65138-v • 06:38 min • December 8th, 2023
December 8th, 2023 •
1, 1, 1,2, 1,3, 1, 1, 1, 1,4, 1, 1
1Bacterial Pathogenesis Laboratory, Infectious Diseases and Immunology Group, Translational Health Science and Technology Institute, NCR Biotech Science Cluster, 2Clinical Microbiology Division, CSIR-Indian Institute of Integrative Medicine, 3ICAR-Research Complex for Eastern Region, 4Public Health Research Institute, Rutgers University

Chapters

Summary
Automatic Translation

This protocol details the enrichment of native mycobacterial extracellular vesicles (mEVs) from axenic cultures of Mycobacterium smegmatis (Msm) and how mCherry (a red fluorescent reporter)-containing recombinant MsmEVs can be designed and enriched. Lastly, it verifies the novel approach with the enrichment of MsmEVs containing the EsxA protein of Mycobacterium tuberculosis.

Tags

Immunology and Infection Extracellular vesicles recombinant vesicles Esat-6 mycobacteria ExsA mCherry Cfp-29 bacteria
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter