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Journal / Immunology and Infection / 마이코박테리아의 native and recombinant extracellular…
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Immunology and Infection

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JoVE Journal Immunology and Infection
마이코박테리아의 native and recombinant extracellular vesicles 농축
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마이코박테리아의 native and recombinant extracellular vesicles 농축

Article DOI: 10.3791/65138-v • 06:38 min • December 8th, 2023
December 8th, 2023 •
1, 1, 1,2, 1,3, 1, 1, 1, 1,4, 1, 1
1Bacterial Pathogenesis Laboratory, Infectious Diseases and Immunology Group, Translational Health Science and Technology Institute, NCR Biotech Science Cluster, 2Clinical Microbiology Division, CSIR-Indian Institute of Integrative Medicine, 3ICAR-Research Complex for Eastern Region, 4Public Health Research Institute, Rutgers University

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이 프로토콜은 Mycobacterium smegmatis(Msm)의 액센 배양에서 천연 마이코박테리아 세포외 소포체(mEV)의 농축과 mCherry(적색 형광 리포터) 함유 재조합 MsmEV를 설계하고 농축할 수 있는 방법을 자세히 설명합니다. 마지막으로, 결핵균(Mycobacterium tuberculosis)의 EsxA 단백질을 함유한 MsmEV의 농축을 통해 새로운 접근법을 검증합니다.

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Immunology and Infection 세포외 소포 재조합 소포 Esat-6 마이코박테리아 ExsA mCherry Cfp-29 박테리아
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