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Transpupillary-Guided Trans-Scleral Transplantation of Subretinal Grafts in a Retinal Degeneration Mouse Model

Article DOI: 10.3791/65448-v • 07:37 min • January 26th, 2024
January 26th, 2024 •
1, 1, 1, 1, 1, 1, 1, 1,2
1Wilmer Eye Institute, Johns Hopkins University School of Medicine, 2Department of Genetic Medicine, Johns Hopkins University School of Medicine

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Summary
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This protocol presents a transpupillary vision-guided trans-scleral approach to safely and precisely deliver subretinal cellular grafts, with a low rate of surgical complications, in mouse recipients with or without retinal degeneration.

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subretinal transplantation photoreceptor retinal degeneration age-related macular degeneration retinitis pigmentosa stem cell retinal organoid
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