Immunology and Infection
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通过离体活细胞成像询问唾液腺中的细胞间相互作用
Summary November 17th, 2023
Please note that all translations are automatically generated.
Click here for the English version.
免疫荧光成像受到仅在单个快照中观察复杂、时间依赖性生物过程的能力的限制。本研究概述了对精密切割小鼠下颌下腺切片进行的实时成像方法。这种方法可以实时观察体内平衡过程中的细胞间相互作用以及再生和修复过程。
Transcript
我的研究深入探讨了免疫系统在再生和修复中的作用。我专注于了解巨噬细胞在唾液腺再生损伤过程中如何与上皮祖细胞相互作用,以及这种相互作用如何塑造修复过程。在免疫学和再生医学领域,爱丁堡大学最近的临床试验表明,递送巨噬细胞作为肝银屑病的治疗方法可有效减少肝脏瘢痕形成,并且是治疗肝衰竭的可行方法。
使用离体精密切片培养模型与荧光标记细胞的实时成像相结合,使我们能够实时探索细胞间的相互作用,但处于与体内组织非常相似的状态。了解损伤后巨噬细胞的定位,可以让我们了解损伤后巨噬细胞优先与哪些细胞类型相互作用。这最终使我们能够使用其他方法,例如单细胞RNA测序分析,以尝试解开这种相互作用的分子基础,从而为未来的治疗选择提供信息。
我们将探索巨噬细胞和上皮细胞之间的双向信号传导,并测试唾液腺稳态是否可以通过外源性替换这些缺失的信号来挽救。
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