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Studying Spatial Protein Expression Using Agarose Embedded Lung Tissue Sections (Video) | JoVE

Innovative Lung Imaging Using Agarose-Embedded Vibratome Sections

Article DOI: 10.3791/65943-v • 01:35 min • October 6th, 2023
October 6th, 2023 •
1,2,3, 1,2,3, 1,2,3,4
1Wallenberg Centre for Molecular Medicine, Lund University, 2Department of Clinical Sciences, Lund University, 3Lund Stem Cell Centre, Lund University, 4Department of Cardiothoracic Surgery and Transplantation, Skåne University Hospital

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Automated Vibratome Sectioning Agarose-embedded Lung Tissue Multiplex Fluorescence Imaging Lung Tissue Processing Paraffin Embedding OCT Embedding Microtome Cryostat Precision-cut Lung Slices Primary Lung Tissue Culture Advanced Light Imaging Readouts Reproducible Imaging Antibodies Species Compatibility Tissue-processing Pipeline Fixed Lung Tissue Automated Vibratome Sectioning Lung Sections Thickness Antigen Retrieval Native Tissue Preservation Spatial Protein Expression Quantification Lung Injury And Regeneration
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