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 JoVE Biology

Mouse Dorsal Forebrain Explant Isolation

1, 2, 3

1Department of Developmental and Cell Biology, University of California, Irvine (UCI), 2Department of Physiology and Biophysics, University of California, Irvine (UCI), 3Department of Pathology, University of California, Irvine (UCI)

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    Summary

    This video demonstrates the protocol for isolating and culturing explants of the mouse forebrain from embyonic day 12 mice. Procedures for removal of the uterus, embryos from uterus, and dissection of embryos are given. In addition the methodology for transferring these explants onto specialized membranes on which they are cultured is demonstrated. The development of the forebrain can be studied in vitro using this preparations as well as changes in gene expression.

    Date Published: 1/30/2007, Issue 2; doi: 10.3791/135

    Cite this Article

    Currle, S., Kolski-Andreaco, A., Monuki, E. S. Mouse Dorsal Forebrain Explant Isolation. J. Vis. Exp. (2), e135, doi:10.3791/135 (2007).

    Disclosures

    The authors have nothing to disclose.

    Comments

    2 Comments

    What kind of membrane is this? Will neurons grow out of the explant?
    Reply

    Posted by: AnonymousJuly 22, 2008, 3:37 AM

    Whatman Nucleopore filter. Don't know about the neurons (we don't use this system for neuronal process outgrowth). ESM
    Reply

    Posted by: AnonymousJuly 23, 2008, 2:18 AM

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