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A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization
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A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization

Article DOI: 10.3791/1395-v • 11:02 min • July 6th, 2009
July 6th, 2009 •
1, 2
1Molecular and Cellular Biology, University of California, Davis, 2Division of Basic Science, Fred Hutchinson Cancer Research Center - FHCRC

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Summary
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This is a high-throughput sperm cryopreservation protocol for zebrafish. Sperm cryopreserved using this protocol has an average of 25% fertility in subsequent vitro fertilization and is stable over many years.

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Zebrafish Sperm Cryopreservation In Vitro Fertilization High-throughput Method Harvey Method Adaptation Sample Uniformity Freezing Media Cryoprotectant Cryovials Sperm Freezing Sperm Thawing Capillary Tubes Team Work Fertility Rate
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