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Lentivirus Production
JoVE Journal
Biology
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JoVE Journal Biology
Lentivirus Production
DOI:

11:42 min

October 02, 2009

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Chapters

  • 00:00Title
  • 00:46Introduction
  • 01:15Transfection of 293 Cells To Produce Lentivirus
  • 03:24Lentivirus Harvest
  • 04:39Lentivirus Concentration via Ultracentrifugation
  • 07:24Lentivirus Titration Using Flow Cytometry
  • 11:17Conclusion

Summary

Automatic Translation

To make lentiviruses, DNA vectors are transfected into human 293 cells. After harvest and concentrating the supernatant, virus titer is determined by fluorescence expression with a flow cytometer.

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