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Feeder-Free Adaptation, Culture and Passaging of Human IPS Cells using Complete KnockOut Serum Replacement Feeder-Free Medium
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Feeder-Free Adaptation, Culture and Passaging of Human IPS Cells using Complete KnockOut Serum Replacement Feeder-Free Medium

Article DOI: 10.3791/2236-v • 08:30 min • July 15th, 2010
July 15th, 2010 •
1, 1
1GIBCO, Life Technologies

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The following protocol provides instruction for adapting human induced Pluripotent Stem (iPS) Cells to feeder-free culture using complete KnockOut Serum Replacement Feeder-Free medium (KSR-FF). Once adapted, instructions for continual maintenance are also provided.

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Feeder-Free Adaptation Culture Passaging Human IPS Cells Complete KnockOut Serum Replacement Feeder-Free Medium Reprogrammed IPS Cells Embryonic Stem Cells Pluripotent Cells Drug Discovery Cell Therapy Basic Research GIBCO Media And Reagents Knockout DMEM Knockout Serum Replacement IPS Cell Culture Feeder-free Culture Knockout SR Growth Factor Cocktail Traditional Culture Methods Mouse Fibroblast Feeder Layers Human Fibroblast Feeder Layers Feeder-conditioned Medium Labor-intensive Hard To Scale Undifferentiated HiPS Cells Undefined Conditions
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