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Application Notes

Simplified LC/MS/MS Bioanalytical Method Development with Radar Technology - ADVERTISEMENT

Robert S. Plumb1
1Pharmaceutical Business Operations, Waters Corporation

Abstract

The development of a bioanalytical assay requires the development of a MRM method as well as a chromatography method, which requires positioning of the analyte peak of interest away from the endogenous background signal. The Xevo™ TQ-S tandem quadrupole mass spectrometer with RADAR™ Technology allows the simultaneous collection of full scan and MS/MS MRM data, vastly simplifying the method development process.

For a bioanalysis method to be effective it must be transferable, reproducible, and robust. The development of a reliable LC/MS/MS bioanalysis assay involves the thorough optimization of sample preparation, MS detection, and chromatography conditions. This can often be a time-consuming process, requiring the analyte peaks(s) be resolved from endogenous matrix components that cause ion suppression and assay irreproducibility. This often requires multiple analytical runs to acquire the necessary MRM, product ion, and full scan data.

Described here is the use of Xevo™ TQ-S Mass Spectrometer with RADAR™ Technology - a novel dual-scan data collection capability to simplify bioanalysis method development. The Xevo TQ-S employs a novel collision cell design which allows for the simultaneous collection of full scan MS and MRM data.

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