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Purificazione di Hsp104, una proteina Disaggregase
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Purificazione di Hsp104, una proteina Disaggregase

Article DOI: 10.3791/3190-v • 07:18 min • September 30th, 2011
September 30th, 2011 •
1, 1, 1
1Department of Biochemistry and Biophysics, University of Pennsylvania

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Qui, descriviamo un protocollo per la purificazione del altamente attivi Hsp104, un esamerica AAA + proteine ​​da lievito, che le coppie idrolisi dell'ATP per disaggregazione delle proteine. Questo schema sfrutta un costrutto His6-tag per la purificazione di affinità

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Biologia Molecolare Numero 55 Neuroscienze Hsp104 AAA + disaggregase shock termico amiloide prione
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