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 JoVE Bioengineering

Ensayos Mecánicos del ratón arterias carótidas: desde recién nacidos hasta adultos

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Department of Biomedical Engineering, Saint Louis University

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Cite this Article: Ensayos Mecánicos del ratón arterias carótidas: desde recién nacidos hasta adultos

Amin, M., Le, V. P., Wagenseil, J. E. Mechanical Testing of Mouse Carotid Arteries: from Newborn to Adult. J. Vis. Exp. (60), e3733, doi:10.3791/3733 (2012).

Disclosures: Ensayos Mecánicos del ratón arterias carótidas: desde recién nacidos hasta adultos

Materials: Ensayos Mecánicos del ratón arterias carótidas: desde recién nacidos hasta adultos

Name Company Catalog Number Comments
Air tank and regulator Airgas UN3156 For pressurizing myograph
Pressure myograph and software Danish Myotechnology 110P, MyoView With custom cannulae (Figure 2)
Inverted microscope, 5x lens and camera Carl Zeiss, Inc. Axiovert 40C For tracking artery diameter
Physiological saline solution (PSS) Sigma-Aldrich Recipe and details in Table 2
Surgical tape Various For securing the mouse during dissection
Dissection board Fisher Scientific 09-002-24A For securing mouse during dissection
Dissecting microscope with camera Carl Zeiss, Inc. Stemi 2000-C For arterial dissection and mounting
Dissecting scissors Fine Science Tools 14058-11 For cutting skin and opening the chest
Fine tweezers (2) Fine Science Tools 11200-14 For grasping artery ends
Curved forceps Fine Science Tools 11274-20 For clearing tissue and exposing carotid arteries
Micro-scissors Fine Science Tools 15005-08 For precise cutting of arteries
7-0 and 10-0 silk suture Various For estimating length and fastening arteries on cannulae
Digital calipers Fisher Scientific 806-93-111 For measuring suture length and checking artery length
Disposable scalpel Feather Safety Razor Co, Ltd. No. 15 For cutting artery rings
Activated charcoal Sigma-Aldrich C4386-500G For marking cut locations on vessels
18G Needle BD Biosciences 305136 For applying activated charcoal to vessels, clearing blood and filling myograph tubing
20 mL syringe Various For clearing blood and filling myograph tubing
Petri dish Fisher Scientific 08-757-13B For inserting vessels after dissection and testing to take pictures
Microfuge tube Fisher Scientific 02-682-550 For storing vessels before testing
Fine wire California Fine Wire Company 100192 For clearing clogged cannula
ImageJ software National Institutes of Health www. rsbweb.nih.gov/ij Open-source image processing program developed by NIH
Matlab software Mathworks Useful for analyzing data and fitting constitutive equations

References: Ensayos Mecánicos del ratón arterias carótidas: desde recién nacidos hasta adultos

  1. Dobrin, P.B. Chapter 3: Physiology and Pathophysiology of Blood Vessels. In: The Basic Science of Vascular Disease. Sumpio, B.E., Sidawy, A.N., & DePalma, R.G., eds., Futura Publishing, New York, 69-105 (1997).
  2. Wagenseil, J.E. & Mecham, R.P. Vascular extracellular matrix and arterial mechanics. Physiol. Rev. 89 (3), 957-989 (2009).
  3. Le, V.P., Knutsen, R.H., Mecham, R.P., & Wagenseil, J.E. Decreased aortic diameter and compliance precedes blood pressure increases in postnatal development of elastin-insufficient mice. Am. J. Physiol. Heart Circ. Physiol., (2011).
  4. Pezet, M., et al. Elastin haploinsufficiency induces alternative aging processes in the aorta. Rejuvenation Res. 11 (1), 97-112 (2008).
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  7. Eberth, J.F., et al. Importance of pulsatility in hypertensive carotid artery growth and remodeling. J. Hypertens. 27 (10), 2010-2021 (2009).
  8. Faury, G., et al. Developmental adaptation of the mouse cardiovascular system to elastin haploinsufficiency. J. Clin. Invest. 112 (9), 1419-1428 (2003).
  9. Amin, M., Kunkel, A., Le, V.P., & Wagenseil, J.E. Effects of Storage Duration on the Mechanical Behavior and Morphology of Mouse Carotid Artery. Journal of Biomechanical Engineering., 133 (2011).
  10. Fung, Y.C. Biomechanics : Mechanical Properties of Living Tissues, 2nd ed., Springer-Verlag, New York, (1993).
  11. Wagenseil, J.E., et al. Effects of elastin haploinsufficiency on the mechanical behavior of mouse arteries. Am. J. Physiol. Heart. Circ. Physiol. 289 (3), H1209-1217 (2005).
  12. Chuong, C.J. & Fung, Y.C. On residual stresses in arteries. J. Biomech. Eng. 108 (2), 189-192 (1986).
  13. Fonck, E., et al. Effect of elastin degradation on carotid wall mechanics as assessed by a constituent-based biomechanical model. Am. J. Physiol. Heart. Circ. Physiol. 292 (6), H2754-2763 (2007).
  14. Rezakhaniha, R., Fonck, E., Genoud, C., & Stergiopulos, N. Role of elastin anisotropy in structural strain energy functions of arterial tissue. Biomech. Model Mechanobiol. 10 (4), 599-611 (2011).
  15. Eberth, J.F., Taucer, A.I., Wilson, E., & Humphrey, J.D. Mechanics of carotid arteries in a mouse model of Marfan Syndrome. Ann. Biomed. Eng. 37 (6), 1093-1104 (2009).
  16. Wan, W. Yanagisawa, H., & Gleason, R.L., Jr. Biomechanical and microstructural properties of common carotid arteries from fibulin-5 null mice. Ann. Biomed. Eng. 38 (12), 3605-3617 (2010).
  17. Faury, G., et al. Relation between outer and luminal diameter in cannulated arteries. Am. J. Physiol. 277 (5 Pt. 2), H1745-1753 (1999).
  18. Sonveaux, P. et al. Endothelin-1 is a critical mediator of myogenic tone in tumor arterioles: implications for cancer treatment. Cancer Res. 64 (9), 3209-3214 (2004).
  19. Mulcahy, C., Amin, M., & Wagenseil, J.E. Creation of a finite element model of a mouse artery to examine circumferential stress distribution. Biomedical Engineering Society Annual Meeting. Austin, TX, (2010).

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