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Riprogrammare cellule somatiche umane in cellule staminali pluripotenti indotte (iPSCs) Utilizzo vettore retrovirale con GFP
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Riprogrammare cellule somatiche umane in cellule staminali pluripotenti indotte (iPSCs) Utilizzo vettore retrovirale con GFP

Article DOI: 10.3791/3804-v • 08:25 min • April 3rd, 2012
April 3rd, 2012 •
1, 1, 1
1Yale Stem Cell Center, Department of Genetics, Yale School of Medicine

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Un metodo per generare umani cellule staminali pluripotenti indotte (iPSCs) tramite retrovirus-mediata espressione ectopica di Oct4, Sox2, Klf4 e MYC è descritto. Un modo pratico per identificare umani IPSC colonie sulla base di GFP è anche discusso.

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Biologia delle cellule staminali le cellule umane iPS riprogrammazione vettori retrovirali e pluripotenza
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