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Overvågning plasmidreplikation Live pattedvrceller over flere generationer ved fluorescensmikroskopi
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Overvågning plasmidreplikation Live pattedvrceller over flere generationer ved fluorescensmikroskopi

Article DOI: 10.3791/4305-v • 12:34 min • December 13th, 2012
December 13th, 2012 •
1, 1, 1
1Department of Oncology, University of Wisconsin - Madison

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En fremgangsmåde til at observere de individuelle DNA-molekyler i levende celler er beskrevet. Teknikken er baseret på binding af en fluorescens-mærket lac repressorproteinet til bindingssteder splejset ind DNA'et af interesse. Denne fremgangsmåde kan tilpasses til at følge mange rekombinante DNA'er i levende celler over tid.

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Genetik Molecular Biology Cellular Biology Genomics DNA-syntese DNA partitionering plasmider lac-operatoren lac-repressor pattedyrceller fluorescensmikroskopi
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