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Forberedelse af Mgm101 Recombination Protein af MBP-baserede tagging strategi
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Forberedelse af Mgm101 Recombination Protein af MBP-baserede tagging strategi

Article DOI: 10.3791/50448-v • 11:40 min • June 25th, 2013
June 25th, 2013 •
1, 1, 1, 1
1Department of Biochemistry and Molecular Biology, State University of New York Upstate Medical University

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Gæren mitokondrie nucleoid protein, Mgm101, er en RAD52-typen rekombination protein, som danner store oligomere ringe. En protokol er beskrevet til fremstilling af opløselig rekombinant Mgm101 hjælp maltosebindingsproteinet (MBP)-tagging strategi kombineret med kationbytning og gelpermeationskromatografi.

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Biokemi Genetik Molekylærbiologisk Institut Proteiner Mgm101 RAD52 mitokondrier rekombination mtDNA maltose-bindende protein
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