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Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure
JoVE Journal
Biology
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JoVE Journal Biology
Isolation of Human Hepatocytes by a Two-step Collagenase Perfusion Procedure
DOI:

07:09 min

September 03, 2013

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Chapters

  • 00:05Title
  • 01:26Preparation of Perfusion Equipment and Solutions
  • 02:00Perfusion of the Liver
  • 04:29Isolation of Hepatocytes
  • 06:03Isolated Hepatocyte Viability, Yield and Purity
  • 06:47Conclusion

Summary

Automatic Translation

A modified two-step collagenase perfusion procedure for isolation of human hepatocytes is described. This method can also be applied to other mammalian livers. The isolated hepatocytes are available in high yield and viability, making them a suitable model for scientific research in areas such as liver regeneration, pharmacokinetics and toxicology.

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