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JoVE Journal
Immunology and Infection

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Culturing and Maintaining Clostridium difficile in an Anaerobic Environment
 

Culturing and Maintaining Clostridium difficile in an Anaerobic Environment

Article DOI: 10.3791/50787-v 11:13 min September 14th, 2013
September 14th, 2013

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Summary

Clostridium difficile is a pathogenic bacterium that is a strict anaerobe and causes antibiotic associated diarrhea (AAD). Here, methods for isolating, culturing and maintaining C. difficile vegetative cells and spores are described. These techniques necessitate an anaerobic chamber, which requires regular maintenance to ensure proper conditions for optimal C. difficile cultivation.

Tags

Clostridium Difficile Anaerobic Environment Isolating C. Difficile Culturing C. Difficile Glycerol Stocks Spore Stocks Anaerobic Chamber Optimal C. Difficile Growth Oxygen Contamination Regular Maintenance Efficient Cultivation
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