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JoVE Journal
Biology

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The Cell-based L-Glutathione Protection Assays to Study Endocytosis and Recycling of Plasma Membrane Proteins
 

The Cell-based L-Glutathione Protection Assays to Study Endocytosis and Recycling of Plasma Membrane Proteins

Article DOI: 10.3791/50867-v 09:22 min December 13th, 2013
December 13th, 2013

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Summary

Membrane trafficking involves transport of proteins from the plasma membrane to the cell interior (i.e. endocytosis) followed by trafficking to lysosomes for degradation or to the plasma membrane for recycling. Methods described in this article are designed to study endocytosis and recycling of plasma membrane proteins.

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Cell-based L-glutathione Protection Assays Endocytosis Recycling Plasma Membrane Proteins Membrane Trafficking Protein Receptors Channels Transporters Adhesion Molecules Cell Surface Biotin Labeling N-hydroxysuccinimide (NHS) Ester Temperature Endocytic Trafficking Disulfide Bond Streptavidin Agarose Western Blotting
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