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Coculture System with an Organotypic Brain Slice and 3D Spheroid of Carcinoma Cells

Article DOI: 10.3791/50881-v • 07:48 min • October 9th, 2013
October 9th, 2013 •
1, 1, 2, 1, *3, *1
1Department of Hematology and Oncology, University of Göttingen, 2Institute of Neuropathology, University of Göttingen, 3Institute of Anatomy and Cellbiology, University of Halle
* These authors contributed equally

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Summary
Automatic Translation

The organotypic brain slice coculture with carcinoma cells enables visualizing morphological changes by fluorescence as well as bright field (video) microscopy during the process of carcinoma cell invasion of brain tissue. This model system also allows for cell exchange and replenishment approaches and offers a wide variety of manipulations and analyses.

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Coculture System Organotypic Brain Slice 3D Spheroid Carcinoma Cells Cerebral Metastasis Resident Cells Stromal Cells Microenvironment Tumor-associated Macrophages (TAM) Migration Invasion Proliferation Tissue-specific Macrophages Kupffer Cells Microglia Astrocytes Glial Reactions Brain Metastasis Formation Bright Field Microscopy In Vivo/in Vitro Model Live Imaging Cerebral Colonization Behavior
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