JoVE Journal
Neuroscience
Neuroscience
A subscription to JoVE is required to view this content.
Chapters
Summary
The floor plate is a crucial structure of the developing spinal cord, providing diffusible signals to progenitors and differentiating neurons. We describe a method to produce floor-plate conditioned medium, to apply it to fresh spinal cord tissue, and to assess the consequences on proteins of interest by biochemistry.