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In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices
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In vivo Postnatal Electroporation and Time-lapse Imaging of Neuroblast Migration in Mouse Acute Brain Slices

Article DOI: 10.3791/50905-v • 10:51 min • November 25th, 2013
November 25th, 2013 •
*1, *1, 2, 1, 1
1Wolfson Centre for Age-Related Diseases, King's College London, 2David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology
* These authors contributed equally

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Summary
Automatic Translation

Neuroblast migration is a fundamental event in postnatal neurogenesis. We describe a protocol for efficient labeling of neuroblasts by in vivo postnatal electroporation and subsequent visualization of their migration using time-lapse imaging of acute brain slices. We include a description for the quantitative analysis of neuroblast dynamics by video tracking.

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In Vivo Postnatal Electroporation Time-lapse Imaging Neuroblast Migration Mouse Acute Brain Slices Subventricular Zone (SVZ) Rostral Migratory Stream (RMS) Olfactory Bulb (OB) Molecular Mechanisms Signaling Pathways Neuroblast Motility Therapeutic Regenerative Potential Injury Stroke Degeneration Confocal Spinning Disk Microscopy Brain Slice Cultures GFP-expressing Plasmid
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