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Journal / Biology / Agroinfiltration and PVX Agroinfection in Potato…
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Biology

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JoVE Journal Biology
Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana
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Agroinfiltration and PVX Agroinfection in Potato and Nicotiana benthamiana

Article DOI: 10.3791/50971-v • 07:33 min • January 3rd, 2014
January 3rd, 2014 •
1,2, 1, 1
1Wageningen UR Plant Breeding, Wageningen University, 2Key Laboratory of Horticultural Plant Biology, Huazhong Agricultural University, Ministry of Education, National Centre for Vegetable Improvement (Central China), Potato Engineering and Technology Research Centre of Hubei Province, Huazhong Agricultural University

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Summary
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Agroinfiltration and PVX agroinfection are routine functional assays for transient ectopic expression of genes in plants. These methods are efficient assays in effectoromics strategies (rapid resistance and avirulence gene discovery) and crucial to modern research in molecular plant pathology. They meet the demand for robust high-throughput functional analysis in plants.

Tags

Agroinfiltration PVX Agroinfection Transient Expression Assays Functional Analysis Candidate Genes Plants Agrobacterium Tumefaciens Dicot Plant Species Solanum Tuberosum Solanum Section Petota Nicotiana Benthamiana Resistance Genes Avirulence Genes R-AVR Interactions Host-pathogen Interactions Nonspecific Defense Responses High-throughput Screening
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