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An Engulfment Assay: A Protocol to Assess Interactions Between CNS Phagocytes and Neurons
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
An Engulfment Assay: A Protocol to Assess Interactions Between CNS Phagocytes and Neurons
DOI:

07:38 min

June 08, 2014

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Chapters

  • 00:05Title
  • 01:19Anterograde Labeling of RGC Presynaptic Inputs
  • 03:26Prepare Tissue for Imaging
  • 05:56Results: Strategy to Assess Microglia-mediated Engulfment of RGC Presynaptic Inputs
  • 07:09Conclusion

Summary

Automatic Translation

Microglia are the resident immune cells of the central nervous system (CNS) with a high capacity to phagocytose or engulf material in their extracellular environment. Here, a broadly applicable, reliable, and highly quantitative assay for visualizing and measuring microglia-mediated engulfment of synaptic components is described.

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