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An Engulfment Assay: A Protocol to Assess Interactions Between CNS Phagocytes and Neurons
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Neuroscience
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JoVE Journal Neuroscience
An Engulfment Assay: A Protocol to Assess Interactions Between CNS Phagocytes and Neurons

An Engulfment Assay: A Protocol to Assess Interactions Between CNS Phagocytes and Neurons

DOI:
10.3791/51482-v

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07:38 min

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June 08, 2014

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, , ,
1Department of Neurology, F.M. Kirby Neurobiology Center,Boston Children’s Hospital, Harvard Medical School

Chapters

  • 00:05Title
  • 01:19Anterograde Labeling of RGC Presynaptic Inputs
  • 03:26Prepare Tissue for Imaging
  • 05:56Results: Strategy to Assess Microglia-mediated Engulfment of RGC Presynaptic Inputs
  • 07:09Conclusion

Summary

Automatic Translation

Automatic Translation

Microglia are the resident immune cells of the central nervous system (CNS) with a high capacity to phagocytose or engulf material in their extracellular environment. Here, a broadly applicable, reliable, and highly quantitative assay for visualizing and measuring microglia-mediated engulfment of synaptic components is described.

Tags

PhagocytosisMicrogliaCNSEngulfment AssayNeurodegenerative DiseaseSynaptic PruningRetinogeniculate SystemAstrocytesMacrophagesSynaptic Remodeling

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