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Automated Measurement of Microcirculatory Blood Flow Velocity in Pulmonary Metastases of Rats

Article DOI: 10.3791/51630-v • 10:56 min • November 30th, 2014
November 30th, 2014 •
*1, *2, 2, 2, 2, 2, 2, 3, 2, 4
1Division of Plastic, Maxillofacial, and Oral Surgery, Duke University Medical Center, 2Department of Radiation Oncology, Duke University Medical Center, 3Department of Cardiology, University of Colorado Denver, 4Department of Physical Chemistry, University of Mainz
* These authors contributed equally

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Summary
Automatic Translation

A method is presented to measure microcirculatory blood flow velocity in pulmonary cancer metastases of the pleural surface in rats in an automated fashion, using closed-chest pulmonary intravital microscopy. This model has potential to be used as a widespread tool to perform physiologic research on pulmonary metastases in rodents.

Tags

Automated Measurement Microcirculatory Blood Flow Velocity Pulmonary Metastases Rats Animal Models Lung Metastases Intravital Microscopy Functional Imaging Quantifying Microcirculation Closed-chest Pulmonary Window Automated Analysis Blood Flow Velocity And Direction Pharmacological Intervention Tortuous Vasculature Solid Tumors Invasiveness Practicality Widespread Use
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