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JoVE Journal Neuroscience
High-throughput analyse af Mammale Olfaktoriske Receptors
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High-throughput analyse af Mammale Olfaktoriske Receptors: Måling af receptor-aktivering via luciferaseaktivitet

Article DOI: 10.3791/51640-v • 12:02 min • June 2nd, 2014
June 2nd, 2014 •
1, 1, 1
1Monell Chemical Senses Center

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Olfaktoriske receptor aktivering mønstre indkode lugt identitet, men manglen på offentliggjorte data, der identificerer lugtstof ligander for pattedyr olfaktoriske receptorer hindrer udviklingen af ​​en omfattende model af lugt kodning. Denne protokol beskriver en fremgangsmåde til at lette højkapacitetsidentifikation af olfaktoriske receptorligander og kvantificering af receptor-aktivering.

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Neuroscience Firefly luciferase, Dual-Glo Luciferase Assay lugtesansen Olfaktoriske receptor lugtstof GPCR High-throughput
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