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JoVE Journal
Neuroscience

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Isolation of Primary Murine Brain Microvascular Endothelial Cells
 

Isolation of Primary Murine Brain Microvascular Endothelial Cells

Article DOI: 10.3791/52204-v 08:14 min November 14th, 2014
November 14th, 2014

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Summary

Brain microvascular endothelial cells (BMEC) are interconnected by specific junctional proteins forming a highly regulated barrier separating blood and the central nervous system (CNS), the so-called blood-brain-barrier (BBB). The isolation of primary murine brain microvascular endothelial cells, as discussed in this protocol, enables detailed in vitro studies of the BBB.

Tags

Isolation Primary Murine Brain Microvascular Endothelial Cells Blood-brain-barrier Tight Junctions Adherens Junctions Neurovascular Unit CNS Homeostasis Dysfunction Pathogenesis Therapies Animal Models In Vitro Studies
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