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JoVE Journal Developmental Biology
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Zebrafish Keratocyte Explants to Study Collective Cell Migration and Reepithelialization in Cutaneous Wound Healing

Article DOI: 10.3791/52489-v • 09:36 min • February 25th, 2015
February 25th, 2015 •
1, 1, 2, 1, 2, 1
1Biomedical Sciences Program, Midwestern University, 2Department of Microbiology & Immunology, Midwestern University

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Summary
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Zebrafish keratocytes migrate in cell sheets from explants and provide an in vitro model for the study of the mechanisms of collective cell migration in the context of epithelial wound healing. These protocols detail an effective way to establish primary explant cultures for use in collective cell migration assays.

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Zebrafish Keratocyte Collective Cell Migration Reepithelialization Cutaneous Wound Healing Explant Cultures Single Cell Migration Motile Properties Rapid Rates Of Motility Actin-rich Lamellae Perpendicular Actin Cable Cell-cell Adhesions Epithelial To Mesenchymal Transition Wound Healing Genes Inflammation Genes In Vitro Model Gene Expression Changes Mutant Zebrafish Lines Transgenic Zebrafish Lines
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