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Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control
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Development of a Quantitative Recombinase Polymerase Amplification Assay with an Internal Positive Control

Article DOI: 10.3791/52620-v • 08:37 min • March 30th, 2015
March 30th, 2015 •
*1, *1, 1
1Department of Bioengineering, Rice University
* These authors contributed equally

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Summary
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Provided is a protocol for developing a real-time recombinase polymerase amplification assay to quantify initial concentration of DNA samples using either a thermal cycler or a microscope and stage heater. Also described is the development of an internal positive control. Scripts are provided for processing raw real-time fluorescence data.

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Quantitative Recombinase Polymerase Amplification Assay Internal Positive Control Isothermal Amplification Pathogen Detection DNA Sample Concentration Real-time Quantitative Recombinase Polymerase Amplification Assay HIV-1 DNA Quantification Real-time RPA Reactions Internal Positive Control Sequence Co-amplification Standard Curve Construction Unknown Sample Concentration Prediction Assay Performance Assessment Real-time Fluorescence Data Collection Microscope Stage Heater Point-of-care QRPA Assay
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