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Isolation and Propagation of Circulating Tumor Cells from a Mouse Cancer Model
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Isolation and Propagation of Circulating Tumor Cells from a Mouse Cancer Model

Article DOI: 10.3791/52861-v • 05:22 min • October 9th, 2015
October 9th, 2015 •
1,2, 1, 1, 1, 1, 3, 1,2
1Department of Biological Sciences, Hunter College of The City University of New York, 2Departments of Biology and Biochemistry, The Graduate Center of The City University of New York, 3Department of Pathology, Immunology, and Laboratory Medicine, University of Florida

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Summary
Automatic Translation

Circulating tumor cells (CTCs) have been shown to play an important role in tumor metastasis. Here, a method for the isolation and propagation of CTCs from the whole blood of a syngeneic mouse tumor model of hepatocellular carcinoma (HCC) metastasis is described.

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Isolation Propagation Circulating Tumor Cells Mouse Cancer Model Cancer Metastasis CTCs Tumor Size Syngeneic Mouse Model Hepatocellular Carcinoma Molecular Mechanisms Solid Organ Tumor Metastasis Efficient Reproducible Non-invasive Technique Biopsy Complications Whole Blood Samples Examination Characterization Clinical Applications Diagnosis Personalized Targeted Therapy
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