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Facile and Efficient Preparation of Tri-component Fluorescent Glycopolymers via RAFT-controlled Polymerization
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Facile and Efficient Preparation of Tri-component Fluorescent Glycopolymers via RAFT-controlled Polymerization

Article DOI: 10.3791/52922-v • 10:54 min • June 19th, 2015
June 19th, 2015 •
1, 1, 2, 3, 1, 1,4
1Department of Biochemistry, University of Missouri, 2Department of Biological Sciences, University of Missouri, 3Department of Molecular Microbiology & Immunology, University of Missouri, 4Department of Child Health, University of Missouri

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Summary
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An efficient, three-step synthesis of RAFT-based fluorescent glycopolymers, consisting of glycomonomer preparation, copolymerization, and post-modification, is demonstrated. This protocol can be used to prepare RAFT-based statistical glycopolymers with desired structures.

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Tri-component Fluorescent Glycopolymers RAFT-controlled Polymerization Synthetic Glycopolymers Biochemical Research Biomedical Research Facile Synthesis Efficient Synthesis Reversible Addition-fragmentation Chain-transfer (RAFT) Galactose-containing Glycomonomer Lactobionolactone N-(2-aminoethyl) Methacrylamide (AEMA) Glunconamidoethyl Methacrylamide (GAEMA) RAFT-mediated Copolymerization N-(2-hydroxyethyl) Acrylamide Fluorescence Labeling Glycomonomers Aqueous Systems (4-cyanopentanoic Acid)-4-dithiobenzoate Low Dispersities Predictable Copolymer Compositions Reproducibility Carboxyfluorescein Succinimidyl Ester Primary Amine Functional Groups Lectin-binding Specificities Agarose Beads
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