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JoVE Journal Bioengineering
Electrotaxis Studies of Lung Cancer Cells using a Multichannel Dual-electric-field Microfluidic Chip
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Electrotaxis Studies of Lung Cancer Cells using a Multichannel Dual-electric-field Microfluidic Chip

Article DOI: 10.3791/53340-v • 08:35 min • December 29th, 2015
December 29th, 2015 •
1, 1, 1,2,3,4,5
1Research Center for Applied Sciences, Academia Sinica, 2Institute of Biophotonics, National Yang-Ming University, 3Biophotonics & Molecular Imaging Research Center (BMIRC), National Yang-Ming University, 4Department of Mechanical and Mechatronic Engineering, National Taiwan Ocean University, 5Ph.D. Program in Microbial Genomics, National Chung Hsing University

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Summary
Automatic Translation

Many microfluidic devices have been developed for use in the study of electrotaxis. Yet, none of these chips allows the efficient study of the simultaneous chemical and electric-field (EF) effects on cells. We developed a polymethylmethacrylate-based device that offers better-controlled coexisting EF and chemical stimulation for use in electrotaxis research.

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Electrotaxis Lung Cancer Cells Microfluidic Chip Directional Cell Migration Direct Current Electric-field (dcEF) Physiological DcEF Microfluidic Assay Experimental Errors Polymeric Substances Polymethylmethacrylate (PMMA) Polydimethylsiloxane (PDMS) Acrylic Microfluidic Chip Chemical And Electrical Stimulation Multichannel Dual-electric-field (MDF) Chip Simultaneous Effects Test Accuracy
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