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JoVE Journal Neuroscience
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A New Approach that Eliminates Handling for Studying Aggression and the "Loser" Effect in Drosophila melanogaster

Article DOI: 10.3791/53395-v • 07:19 min • December 30th, 2015
December 30th, 2015 •
1, 1, 1
1Department of Neurobiology, Harvard Medical School

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Summary
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During fruit fly fights, the behavioral patterns observed, fight dynamics, and associated learning and memory are influenced by experimental conditions. The protocol presented here describes a novel procedure that entirely eliminates handling of flies during experiments. This improves fight dynamics and allows formation of strong "loser" effects.

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Approach Eliminates Handling Studying Aggression Loser Effect Drosophila Melanogaster Aggressive Behavior Behavioral Patterns Genetic Factors Hormonal Factors Environmental Factors Fighting Experience Learning And Memory Pre-test Handling Experimental Procedures Behavioral Apparatus Negative Geotactic Responses Fight Arenas Removable Plastic Slider Sliding Chamber Doors
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