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High-Resolution Quantitative Immunogold Analysis of Membrane Receptors at Retinal Ribbon Synapses

Article DOI: 10.3791/53547-v • 09:24 min • February 18th, 2016
February 18th, 2016 •
1, 2, 2, 1
1Synaptic Physiology Section, National Institute of Neurological Disorders and Stroke, National Institutes of Health, 2Advanced Imaging Core, National Institute on Deafness and Other Communication Disorders, National Institutes of Health

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The postembedding immunogold method is one of the most effective ways to provide high-resolution analyses of the subcellular localization of specific molecules. Here we describe a protocol to quantitatively analyze glutamate receptors at retinal ribbon synapses.

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High-Resolution Quantitative Immunogold Analysis Membrane Receptors Retinal Ribbon Synapses Experiment Approach Analyze Proteins Location Synapses Circuit Technique Estimate Number Density Rapidity Multiple Proteins Freeze-Substitution Ultra-Thin Sectioning Ron Petralia Ya-Xian Wang NIDCD Advanced Imaging Core Dissect Eyeball Microscope Cornea Lens Vitreous Inter-Retinal Surface Sclera Retina Strips Paraformaldehyde PB Solution Glutaraldehyde Calcium Chloride Cryoprotect Glycerol
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