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Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR
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Simultaneous DNA-RNA Extraction from Coastal Sediments and Quantification of 16S rRNA Genes and Transcripts by Real-time PCR

Article DOI: 10.3791/54067-v • 11:37 min • June 11th, 2016
June 11th, 2016 •
1, 2, 2, 1
1Microbiology, School of Natural Sciences, National University of Ireland Galway, 2School of Biological Sciences, University of Essex

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A protocol to quantify bacterial 16S rRNA genes and transcripts from coastal sediments via real-time PCR is provided. The methodology includes the co-extraction of DNA and RNA; preparation of DNA-free RNA; and 16S rRNA gene and transcript quantification via RT-q-PCR, including standard curve construction.

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Simultaneous DNA-RNA Extraction Coastal Sediments Quantification 16S RRNA Genes Transcripts Real-time PCR High Yield Quality DNA RNA Extraction Molecular Analysis Microbial Abundance Diversity Environment Coextraction Downstream Analysis Quantitative PCR Dr. Enrico Tatti NUI Galway Duncan Sweeney University Of Essex Sediment Samples Bead Lysing Tube CTAB Phosphate Buffer Phenol:chloroform:isoamyl Alcohol Homogenize Sample
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