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JoVE Journal
Developmental Biology

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Embryo Microinjection and Electroporation in the Chordate Ciona intestinalis
 

Embryo Microinjection and Electroporation in the Chordate Ciona intestinalis

Article DOI: 10.3791/54313-v 09:38 min October 16th, 2016
October 16th, 2016

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Summary

We present transient transgenesis and gene knockdown in Ciona intestinalis, a chordate sister group to vertebrates, using microinjection and electroporation techniques. Such methods facilitate functional genomics in this simple invertebrate that features rudimentary characteristics of vertebrates, including notochord and head sensory epithelia, and many orthologs of human disease associated genes.

Tags

Embryo Microinjection Electroporation Ciona Intestinalis Gene Regulation Molecular Genetics Chordate Developmental Program Stem Cell Research Drug Discovery Embryo Electroporation Synchronized Embryos Fragile Embryos Dechorionation Micro-injection Technique Ciona Adults Oviduct Artificial Sea Water
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