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Journal / Immunology and Infection / In vitro differentiering af humane CD4 + Foxp3 +…
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Immunology and Infection

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JoVE Journal Immunology and Infection
In vitro differentiering af humane CD4 + Foxp3 + Induced Regulatory T-celler (iTregs) fra naive CD4 + T-celler under anvendelse et TGF-β-holdige protokol
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In vitro differentiering af humane CD4 + Foxp3 + Induced Regulatory T-celler (iTregs) fra naive CD4 + T-celler under anvendelse et TGF-β-holdige protokol

Article DOI: 10.3791/55015-v • 08:20 min • December 30th, 2016
December 30th, 2016 •
1, *1, *1, 1
1Unit of Computational Medicine, Center for Molecular Medicine, Department of Medicine Solna, Karolinska Institutet, Karolinska University Hospital, & Science for Life Laboratory
* These authors contributed equally

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Denne protokol beskriver den reproducerbare generation og fænotypebestemmelse af humane inducerede regulatoriske T-celler (iTregs) fra naive CD4 + -T-celler in vitro. Forskellige protokoller for Foxp3 induktion muliggøre undersøgelsen af ​​særlige iTreg fænotyper opnået med respektive protokoller.

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Immunologi regulatoriske T-celler Treg CD4 + -T-celler magnetisk celleisolering, cytokiner Foxp3 TGF-β IL-2 intracellulær Flowcytometri QRT-PCR
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