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JoVE Journal
Immunology and Infection

This content is Open Access.

Unravelling the Function of a Bacterial Effector from a Non-cultivable Plant Pathogen Using a Yeast Two-hybrid Screen
 

Unravelling the Function of a Bacterial Effector from a Non-cultivable Plant Pathogen Using a Yeast Two-hybrid Screen

Article DOI: 10.3791/55150-v 11:30 min January 20th, 2017
January 20th, 2017

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Summary

Bacterial effector proteins are important for establishing successful infections. This protocol describes the experimental identification of proteinaceous binding partners of a bacterial effector protein in its natural plant host. Identifying these effector interactions via yeast two-hybrid screens has become an important tool in unravelling molecular pathogenicity strategies.

Tags

Bacterial Effector Non-cultivable Plant Pathogen Yeast Two-hybrid Screen Candidatus Phytoplasma Malefactor Protein Malus Domestica Apple Proliferation Disease Molecular Mechanisms Infection Research Infected Trees Control Trees Root Samples Thermal Packs Sterilized Scalpel
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