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JoVE Journal Neuroscience
A Neurosphere Assay to Evaluate Endogenous Neural Stem Cell Activation in a Mouse Model of Minimal Spinal Cord Injury
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A Neurosphere Assay to Evaluate Endogenous Neural Stem Cell Activation in a Mouse Model of Minimal Spinal Cord Injury

Article DOI: 10.3791/57727-v • 09:08 min • September 13th, 2018
September 13th, 2018 •
1,2, 2, 1,2,3
1Institute of Medical Science, University of Toronto, 2Department of Surgery, University of Toronto, 3Institute of Biomaterials and Biomedical Engineering, University of Toronto

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Summary
Automatic Translation

Here, we demonstrate the performance of a minimal spinal cord injury model in an adult mouse that spares the central canal niche housing endogenous neural stem cells (NSCs). We show how the neurosphere assay can be used to quantify activation and migration of definitive and primitive NSCs following injury.

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Neurosphere Assay Endogenous Neural Stem Cell Activation Mouse Model Minimal Spinal Cord Injury Regenerative Medicine Neural Precursor Cells Spinal Cord Injury Effect Surgical Procedure Dissection Steps Precision Hair Clippers Stereotactic Device Rolled Gauze Thoracic Spine Support Laboratory Tape Disinfecting Skin Scalpel Blade Vertical Incision
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