July 2016: This Month in JoVE

1, 2

1Department of Ophthalmology, Massachusetts Eye and Ear, 2JoVE Content Production

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    Summary

    Date Published: 7/08/2016, Issue 113; doi: 10.3791/5802

    Cite this Article

    Chao, W., Kolski-Andreaco, A. July 2016: This Month in JoVE. J. Vis. Exp. (113), e5802, doi:10.3791/5802 (2016).

    Abstract

    Here's a look at what's coming up in the July 2016 issue of JoVE: The Journal of Visualized Experiments.

    In JoVE Neuroscience, gustatory perception - or taste - is an important factor for pollinating insects and the flowers that they feed from. Most studies have used restrained honeybees to study gustatory responses towards nutrients and toxins. This month, Ma et al. present a new behavioral assay that uses freely moving bumblebees to measure how different compounds influence their feeding behavior. This assay will be useful to pollination biologists, toxicologists, and neuroethologists studying the bumblebee's taste system.

    In JoVE Environment, trace metal measurements in natural waters are often inaccurate due to inadequate sampling and analytical techniques. In fact, using improved techniques, researchers are finding that true concentrations of dissolved trace metals may be orders of magnitude lower than previously thought. So Jiann et al. present a protocol for clean sampling and trace metal analysis of river and estuary waters. They present techniques for reducing contamination throughout all phases of trace metal analysis. The improved data quality allows accurate assessment of trace metals and their relationships to environmental parameters.

    In JoVE Engineering, Janus colloids are special nanoparticles that have multiple chemical, physical, and structural properties - making them attractive tools for biomedical applications. Campbell et al. present a method to prepare catalytically active Janus colloids that "swim" in fluids, and determine their 3D motion using fluorescence microscopy. With this method, 3D trajectories are obtained for the swimming colloid, which allows accurate measurement of swimming velocity and other physical phenomena.

    In JoVE Behavior, vision problems can have a major impact on development. But in young children or those with intellectual disabilities, it is often difficult quantitatively assess visual problems, which limits accurate diagnostics. To overcome these problems, Kooiker et al. describe a method for quantifying visual information processing. A remote eye tracker measures eye movements in response to different visual stimuli - providing valuable information for vision assessment and rehabilitation.

    You've just had a sneak peek of the July 2016 issue of JoVE. Visit the website to see the full-length articles, plus many more, in JoVE: The Journal of Visualized Experiments.

    Protocol

    A Novel Behavioral Assay to Investigate Gustatory Responses of Individual, Freely-moving Bumble Bees (Bombus terrestris)

    Carolyn Ma, Sébastien Kessler, Alexander Simpson, Geraldine A. Wright

    Institute of Neuroscience, Newcastle University

    A novel behavioral assay is described for investigating the short term gustatory responses of the mouthparts of freely-moving bumble bees (Bombus terrestris) toward nutrients and toxins in solution

    Clean Sampling and Analysis of River and Estuarine Waters for Trace Metal Studies

    Kuo-Tung Jiann*1, Liang-Saw Wen*2,3, Peter H. Santschi4

    1Department of Oceanography, National Sun Yat-sen University, 2Institute of Oceanography, National Taiwan University, 3Taiwan Ocean Research Institute, National Research Laboratories, 4Department of Oceanography and Marine Sciences, Texas A&M University at Galveston

    Special care using "clean techniques" is required to properly collect and process water samples for trace metal studies in aquatic environments. A protocol for sampling, processing, and analytical procedures with the aim of obtaining reliable environmental monitoring data and results with high sensitivity for detailed trace metal studies is presented.

    Preparation and 3D Tracking of Catalytic Swimming Devices

    Andrew Campbell, Richard Archer, Stephen Ebbens

    Department of Chemical and Biological Engineering, University of Sheffield

    A method to prepare catalytically active Janus colloids that can "swim" in fluids and determine their 3D trajectories is presented.

    A Method to Quantify Visual Information Processing in Children Using Eye Tracking

    Marlou J.G. Kooiker1, Johan J.M. Pel1, Sanny P. van der Steen-Kant2, Johannes van der Steen1,2

    1Department of Neuroscience, Erasmus MC, 2KEI, Royal Dutch Visio

    A method is described to quantify the quality of visual information processing based on reflexive eye movements in response to specific visual modalities. Reaction times and fixation output parameters are used to characterize visual performance in children with and without visual impairments from 6 months of age.

    Disclosures

    No conflicts of interest declared.

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