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Journal / Bioengineering / Production of E. coli-expressed Self-Assembling…
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Bioengineering

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JoVE Journal Bioengineering
Production of E. coli-expressed Self-Assembling Protein Nanoparticles for Vaccines Requiring Trimeric Epitope Presentation
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Production of E. coli-expressed Self-Assembling Protein Nanoparticles for Vaccines Requiring Trimeric Epitope Presentation

Article DOI: 10.3791/60103-v • 10:58 min • August 21st, 2019
August 21st, 2019 •
1,2, 3, 1, 1,2
1U.S. Military HIV Research Program, Walter Reed Army Institute of Research, 2Henry M. Jackson Foundation for the Advancement of Military Medicine, 3Alpha-O Peptides AG

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Summary
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A detailed method is provided here describing the purification, refolding, and characterization of self-assembling protein nanoparticles (SAPNs) for use in vaccine development.

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E. Coli-expressed Self-Assembling Protein Nanoparticles Vaccines Trimeric Epitope Presentation SAPNs Infectious Diseases Purify Refold Characterize Six-helix Bundle Trimeric Antigens Native-like Conformation Innovative SAPN Vaccine Constructs Large-scale Production Clinical Trials Lysis Sonication Imidazole-free Buffer Centrifuge Cellular Lysate Clarified Supernatant Sterile 150-milliliter Flask FPLC Software New Method Option Column Position Dropdown Menu Affinity Technique Column Type Dropdown Menu HisTrap HP Column
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