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Bulk Droplet Vitrification for Primary Hepatocyte Preservation

Article DOI: 10.3791/60250-v • 11:07 min • October 25th, 2019
October 25th, 2019 •
1,2,3, 1,2, 1,2, 1,2, 1,2, 3, 1,2, 1,2, 1,2
1Center for Engineering in Medicine, Harvard Medical School, Massachusetts General Hospital, 2Shriners Hospitals for Children, Boston, 3Department of Surgery, University of Amsterdam

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Summary
Automatic Translation

This manuscript describes an ice-free cryopreservation method for large quantities of rat hepatocytes whereby primary cells are pre-incubated with cryoprotective agents at a low concentration and vitrified in large droplets.

Tags

Bulk Droplet Vitrification Primary Hepatocyte Preservation Cryoprotective Agents Rapid Osmotic Dehydration Low CPA Concentration Vitrification Solution BSA University Of Wisconsin Solution Cryopreservation Hepatocytes Transplantation Bio-artificial Liver Devices Cell Types Cryopreservation Blood Products Stem-cells
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