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Immunology and Infection

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TurboID-Based Proximity Labeling for In Planta Identification of Protein-Protein Interaction Networks

Article DOI: 10.3791/60728-v • 07:02 min • May 17th, 2020
May 17th, 2020 •
*1, *2,3, 1, 1, 2, 2,3
1State Key Laboratory of Agro-Biotechnology and Ministry of Agriculture Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, 2Department of Plant Biology, College of Biological Sciences, University of California, Davis, 3Genome Center, College of Biological Sciences, University of California, Davis
* These authors contributed equally

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Summary
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Described here is a proximity labeling method for identification of interaction partners of the TIR domain of the NLR immune receptor in Nicotiana benthamiana leaf tissue. Also provided is a detailed protocol for the identification of interactions between other proteins of interest using this technique in Nicotiana and other plant species.

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TurboID-based Proximity Labeling Protein-protein Interaction Networks NLR Immune Receptors Plant Defense Nicotiana Benthamiana Toll/interleukin-1 Receptor Domain Protein-protein Interactions Transient Interactions Weak Interactions In Vivo Labeling TurboID Fusions Agrobacterium Tumefaciens Competent Cells Agroinfiltration Abaxial Epidermis Biotin Labeling
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