Department of Molecular Biology, Princeton University
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Hafer, N., Schedl, P. Dissection of Larval CNS in Drosophila Melanogaster. J. Vis. Exp. (1), e85, doi:10.3791/85 (2006).
This video demonstrates how to dissect the CNS from third instar Drosophila larvae. After dissection, the CNS can be stained using a standard immunohistochemistry protocol. Currently, much is still unknown about how the adult nervous system is generated as well as how it stores and transmits information. Studies of the developing Drosophila larval CNS should enhance our knowledge of nervous system wiring and function.
Drosophila larvae exhibit a number of stereotpyed behaviors, such as a response to environmental cues. How are these behaviors learned and stored in the nervous system? Studies of the nervous system during larval development will help to answer this question.
At this stage in Drosophila development, the organism is preparing to undergo the vast morphological changes that occur during pupation. During this time, massive remodeling of the neuromuscular system occurs as the organsim transitions from a crawling maggot into an adult fly. It will be interesting to determine how neurons change during this stage of development and if the same type of behaviors can be observed in the neurons of higher animals.
The authors have nothing to disclose.
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