In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts

Marie Cross; Maureen Powers

doi:10.3791/908

Cite this Article: In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts

Cross, M., Powers, M. In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts. J. Vis. Exp. (19), e908, doi:10.3791/908 (2008).

Abstract: In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts

Nuclear membrane assembly is an essential step in the cell division cycle; this process can be replicated in the test tube by combining Xenopus sperm chromatin, cytosol, and light membrane fractions. Complete nuclei are formed, including nuclear membranes with pore complexes, and these reconstituted nuclei are capable of normal nuclear processes.

Protocol: In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts

The complete text protocol for this experimental approach is available in Current Protocols in Cellular Biology.

Disclosures: In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts

The authors have nothing to disclose.

Ask the Author: In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts

2 Comments

is good

congratulations for you job, i am mexico

Posted by: AnonymousSeptember 3, 2008, 12:26 AM

How to see references???

Posted by: AnonymousSeptember 7, 2008, 10:08 PM

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Date Published	9/02/2008
JoVE Section	General
JoVE Issue	19
Comments	2 Comments
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DOI	doi:10.3791/908